Hani Sitti Nuroniah
Ph.D candidate
Forest Genetics and Forest Tree Breeding
Büsgen Institute
Georg-August University Göttingen
Büsgenweg 2
D-37077 Göttingen
E-mail: hnuroni@gwdg.de
Personal data – education path
1971 born in Bandung, Indonesia
1990-1996 Bachelor of Science- Biology,, Bandung Institute of Technology, Indonesia
1996-1999 Master of Science – Biology, Bandung Institute of Technology, Indonesia
2006 to present PhD student
Experience
1998-2005 Associate Assistant Researcher, Research and Development Center for Forest and Nature Conservation
2004-2005 Technical cooperation project for the promotion of mass propagation technique of
native tree species for reforestation and rehabilitation
1999-2004 The Meranti Nursery Project, Collaboration research project between Forestry
Research and Development Agency and Research Division Komatsu Ltd. of Japan
Research interest
Forest Genetics resources management
Silviculture of dipterocarp species
Selected ongoing or recently completed research projects
Diagostic marker for the identification of tree species and the geographic origin of Shorea leprosula and S. parvifolia
Mass propagation technique of dipterocarp species
Characterization of oil palm (Elaeis guineensis jacq.) microsatelllites
Stuudy of genetic diversity oftrue potato seed (Solanum tuberosum L.) using RAPD
Publication
Sakai, C., Subiakto, A., Nuroniah, H.S., Kamata, N. and Nakamura K. 2002. Mass propagation method from the cutting of three dipterocarps species. J. For. Res 7: 73-80
Project
Shorea leprosula and S. parvifolia (Dipterocarpaceae) are the most common and widespread emergents of lowland dipterocarp forests in Indonesia. This Dipterocarps wood are very important timber in Indonesia with vernacular name “meranti merah”.
The development of tools to test the origin of wood has considerable importance for practical application within the context of forest certification. A protocol to amplify DNA from dry wood has been developed (Rachmayanti et al., 2006). This has led to the development of genetic markers that can be used on dry wood.
My research project is the continuity of the results from previous findings of the Forest Genetics and Forest Tree Breeding Department. In a recent AFLP based study on the genetic diversity of Shorea leprosula and S. parvifolia (Cao et al., 2006), it was found that some AFLP markers could potentially be used as diagnostic markers to differentiate wood origin. There were also other markers that were potentially supposed to verify species S. leprosula and S. parvifolia.
The conversion of AFLP (Amplified Fragment Length Polymorphism) markers from multilocus fingerprints to single locus PCR markers made these potential markers useful as diagnostic markers. Bands of interest may be excised, cloned and transformed into SCAR (Sequence Characterized Amplified Region) marker by designing specific primers.